MARC Record from Library of Congress

LEADER: 04063cam a2200493 i 4500
001 2015935079
003 DLC
005 20151215090917.0
008 150303s2015 nyua b 001 0 eng c
010 $a 2015935079
016 7 $a101662216$2DNLM
020 $a9781493925957 (alk. paper)
020 $a1493925954 (alk. paper)
020 $z9781493925964 (eBook)
020 $z1493925962 (eBook)
035 $a(OCoLC)ocn903596278
040 $aNLM$beng$cNLM$erda$dYDXCP$dNUI$dIDL$dOCLCO$dDLC
042 $apcc
050 00 $aQP624$b.D174526 2015
050 14 $aQH506$b.M45 v.1300
060 00 $aW1$bME9616J v.1300 2015
060 10 $aQU 475
082 00 $a572.8/645$223
130 0 $aDNA replication (Vengrova)
245 10 $aDNA replication :$bmethods and protocols /$cedited by Sonya Vengrova, Coventry, UK, Jacob Dalgaard, Warwick Medical School, University of Warwick, Coventry, UK.
250 $aSecond edition.
264 1 $aNew York :$bHumana Press,$c[2015]
300 $ax, 295 pages :$billustrations ;$c27 cm
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
490 1 $aMethods in molecular biology,$x1064-3745 ;$v1300
490 1 $aSpringer protocols
504 $aIncludes bibliographical references and index.
505 0 $aA High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analyzing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast -- EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection and Sequencing of Okazaki Fragments In S. cerevisiae -- ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin -- Chromatin Immunoprecipitation to Detect DNA Replication and Repair Factors -- Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism -- Single-Molecule Observation of Prokaryotic DNA Replication -- Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.
520 $aUpdated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.
650 0 $aDNA replication$xResearch$xMethodology.
650 0 $aDNA replication$vLaboratory manuals.
650 12 $aDNA Replication.
655 7 $aLaboratory Manuals.$2mesh
700 1 $aVengrova, Sonya,$eeditor.
700 1 $aDalgaard, Jacob Z.,$eeditor.
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$v1300.$x1064-3745
830 0 $aSpringer protocols (Series)$x1949-2448