MARC Record from marc_columbia

LEADER: 06292cam a2200493Ia 4500
001 6988885
005 20221130201114.0
008 080829t20092009nyua b 001 0 eng d
010 $a 2008938341
020 $a9781588298591 (alk. paper)
020 $a1588298590 (alk. paper)
035 $a(OCoLC)ocn244765671
035 $a(OCoLC)244765671
035 $a(NNC)6988885
035 $a6988885
040 $aBTCTA$cBTCTA$dYDXCP$dCUS$dCDX$dCOU$dOrLoB-B
050 4 $aQH506$b.M562 v.485 2009 [QR414.6]
060 4 $aWC 503.6 H6765 2009
082 04 $a616.9'792'0072$222
245 00 $aHIV protocols /$cedited by Vinayaka R. Prasad and Ganjam V. Kalpana.
250 $a2nd ed.
260 $aNew York, NY :$bHumana Press,$c[2009], ©2009.
300 $axiv, 457 pages :$billustrations ;$c27 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
490 1 $aMethods in molecular biology ;$v485
490 1 $aSpringer protocols
504 $aIncludes bibliographical references and index.
505 00 $gSect. I.$tPreparation of Virus Particles and Their Analysis -- $g1.$tMethods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes /$rKimdar Sherefa Kemal, Milan Reinis, Barbara Weiser and Harold Burger -- $g2.$tPurification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies /$rDavid E. Ott -- $g3.$tCalculating HIV-1 Infectious Titre Using a Virtual TCID[subscript 50] Method /$rYong Gao, Immaculate Nankya, Awet Abraha, Ryan M. Troyer, Kenneth N. Nelson, Andrea Rubio and Eric J. Arts -- $gSect. II.$tMethods to Study HIV-1 Replication -- $g4.$tCell-Free Assays for HIV-1 Uncoating /$rChristopher Aiken -- $g5.$tReal-Time PCR Analysis of HIV-1 Replication Postentry Events /$rJean L. Mbisa, Krista A. Delviks-Frankenberry, James A. Thomas, Robert J. Gorelick and Vinay K. Pathak -- $g6.$tAnalysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells /$rDibyakanti Mandal and Vinayaka R. Prasad -- $g7.$tHIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach /$rMichael D. Moore, Mario P. S. Chin and Wei-Shau Hu -- $g8.$tMethods of Preparation and Analysis of Intracellular Reverse Transcription Complexes /$rAriberto Fassati -- $g9.$tAnalysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation /$rSergey N. Iordanskiy and Michael I. Bukrinsky -- $g10.$tIsolation and Analysis of HIV-1 Preintegration Complexes /$rAlan Engelman -- $g11.$tBisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy /$rNathalie J. Arhel and Pierre Charneau -- $g12.$tMethods for the Study of HIV-1 Assembly /$rAbdul A. Waheed, Akira Ono and Eric O. Freed -- $g13.$tAssembly of Immature HIV-1 Capsids Using a Cell-Free System /$rJaisri R. Lingappa and Beth K. Thielen -- $g14.$tPreparation of Recombinant Hiv-1 Gag Protein and Assembly of Virus-Like Particles In Vitro /$rSiddhartha A. K. Datta and Alan Rein -- $g15.$tMethods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides /$rAndrew G. Stephen and Robert J. Fisher -- $g16.$tMethods for Analysis of Incorporation and Annealing of tRNA[superscript Lys] in HIV-1 /$rShan Cen, Fei Guo and Lawrence Kleiman -- $gSect. III.$tSpecialized Approaches to Study HIV-1 Biology and Pathogenesis -- $g17.$tSomatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors /$rSusana T. Valente and Stephen P. Goff -- $g18.$tRapid, Controlled and Intensive Lentiviral Vector-Based RNAi /$rManuel Llano, Natassia Gaznick and Eric M. Poeschla -- $g19.$tReverse Two-Hybrid Screening to Analyze Protein-Protein Interaction of HIV-1 Viral and Cellular Proteins /$rSupratik Das and Ganjam V. Kalpana -- $g20.$tMethods to Study Monocyte Migration Induced by HIV-infected Cells /$rVasudev R. Rao, Eliseo A. Eugenin, Joan W. Berman and Vinayaka R. Prasad -- $g21.$tNovel Mouse Models for Understanding HIV-1 Pathogenesis /$rAviva Joseph, Kaori Sango and Harris Goldstein -- $gSect. IV.$tImmunological Studies of HIV -- $g22.$tMucosal Antibody Responses to HIV /$rZina Moldoveanu and Jiri Mestecky -- $g23.$tIsolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology Assays /$rBarbara L. Shacklett, J. William Critchfield and Donna Lemongello -- $g24.$tQuantifying HIV-1-Specific CD8+ T-Cell Responses Using ELISPOT and Cytokine Flow Cytometry /$rBarbara L. Shacklett, J. William Critchfield and Donna Lemongello -- $g25.$tMultiparameter Flow Cytometry Monitoring of T Cell Responses /$rHolden T. Maecker -- $g26.$tMeasuring HIV Neutralization in a Luciferase Reporter Gene Assay /$rDavid C. Montefiori -- $g27.$tAssessing the Antiviral Activity of HIV-1 -Specific Cytotoxic T Lymphocytes /$rOtto O. Yang -- $g28.$tMethods for Quantitating Antigen-Specific T Cell Responses Using Functional Assays in Rhesus Macaques /$rRama Rao Amara -- $gSect. V.$tDrug Resistant Viruses and Viral Evolution -- $g29.$tIsolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection /$rMaureen Oliveira, Bluma G. Brenner and Mark A. Wainberg -- $g30.$tVirus Evolution as a Tool to Study HIV-1 Biology /$rBen Berkhout and Atze T. Das.
650 0 $aAIDS (Disease)$vLaboratory manuals.
650 0 $aHIV (Viruses)$vLaboratory manuals.
650 0 $aHIV infections$vLaboratory manuals.
650 12 $aHIV-1.$0https://id.nlm.nih.gov/mesh/D015497
650 12 $aHIV Infections.$0https://id.nlm.nih.gov/mesh/D015658
650 22 $aMolecular Biology$xmethods.$0https://id.nlm.nih.gov/mesh/D008967Q000379
650 22 $aImmunologic Techniques.$0https://id.nlm.nih.gov/mesh/D007158
650 22 $aVirology$xmethods.$0https://id.nlm.nih.gov/mesh/D014773Q000379
650 22 $aClinical Protocols.$0https://id.nlm.nih.gov/mesh/D002985
655 2 $aLaboratory Manual.$0https://id.nlm.nih.gov/mesh/D020484
700 1 $aPrasad, Vinayaka R.$0http://id.loc.gov/authorities/names/nb2009002258
700 1 $aKalpana, Ganjam V.$0http://id.loc.gov/authorities/names/nb2009002259
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 485.$0http://id.loc.gov/authorities/names/n92002874
830 0 $aSpringer protocols (Series)$0http://id.loc.gov/authorities/names/no2008180772
852 00 $boff,sci$hQH506$i.M562 v.485 2009g